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Invitrogen

G3BP1 Polyclonal Antibody

高级验证

5个已发表图片

7 篇参考文献

查看其他21个G3BP1抗体

说明书

实验方案

技术支持

货号 PA5-29455

CNY 6,174.00

100 µL

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说明书

实验方案

技术支持

Application

G3BP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

G3BP1 Antibody (PA5-29455) in ICC/IF

Immunofluorescence analysis of G3BP1 was performed using 70% confluent log phase U-2 OS treated with Heat Shock (45 degree celsius, 45 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with G3BP1 Polyclonal Antibody (Product # PA5-29455) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seco... View More

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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G3BP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with G3BP1 specific siRNA and decrease in signal intensity was observed in Western Blot application using Anti-G3BP1 Polyclonal Antibody (Product # PA5-29455). {KD}

产品信息

PA5-29455

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 147 and 466 of Human G3BP1

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.35 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546931

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (90%), Rat (91%), Pig (94%), Chicken (82%), Rhesus Monkey (99%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cytosolic stress granules relieve the ubiquitin-proteasome system in the nuclear compartment.

The EMBO journal

Xu S,Gierisch ME,Schellhaus AK,Poser I,Alberti S,Salomons FA,Dantuma NP

PA5-29455 was used in Western Blot to reveal that stress granules play an important role in the sequestration of cytosolic misfolded proteins, thereby preventing these proteins from accumulating in the nucleus, where they would otherwise infringe nuclear proteostasis.

Wed Feb 01 00:00:00 UTC 2023

Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.

Autophagy

Giovannucci TA,Salomons FA,Stoy H,Herzog LK,Xu S,Qian W,Merino LG,Gierisch ME,Haraldsson M,Lystad AH,Uvell H,Simonsen A,Gustavsson AL,Vallin M,Dantuma NP

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Fri Jul 01 00:00:00 UTC 2022

Small-molecule modulators of INAVA cytosolic condensate and cell-cell junction assemblies.

The Journal of cell biology

Chang D,Luong P,Li Q,LeBarron J,Anderson M,Barrett L,Lencer WI

Published figure using G3BP1 polyclonal antibody (Product # PA5-29455) in Immunocytochemistry

Mon Sep 06 00:00:00 UTC 2021

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.

Cell

Guillén-Boixet J,Kopach A,Holehouse AS,Wittmann S,Jahnel M,Schlüßler R,Kim K,Trussina IREA,Wang J,Mateju D,Poser I,Maharana S,Ruer-Gruß M,Richter D,Zhang X,Chang YT,Guck J,Honigmann A,Mahamid J,Hyman AA,Pappu RV,Alberti S,Franzmann TM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to propose that condensation coupled to conformational rearrangements and heterotypic multivalent interactions may be a general principle underlying RNP granule assembly.

Thu Apr 16 00:00:00 UTC 2020

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.

Cell

Thu Apr 16 00:00:00 UTC 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 UTC 2020

TDP-43, a protein central to amyotrophic lateral sclerosis, is destabilized by tankyrase-1 and -2.

Journal of cell science

McGurk L,Rifai OM,Bonini NM

PA5-29455 was used in Immunocytochemistry-immunoflourescence to study how tankyrase 1 and 2 activity modulates TDP-43 in the disease context of amyotrophic lateral sclerosis.

Tue Jun 23 00:00:00 UTC 2020

Human Insulin Growth Factor 2 mRNA Binding Protein 2 Increases MicroRNA 33a/b Inhibition of Liver ABCA1 Expression and Alters Low-Density Apolipoprotein Levels in Mice.

Molecular and cellular biology

Yang M,Gallo-Ebert C,Hayward M,Liu W,McDonough V,Nickels JT

PA5-29455 was used in Western Blotting to support the hypothesis that IGF2BP2 has an important role in maintaining lipid homeostasis through its modulation of ABCA1 expression, as its overexpression or loss leads to dyslipidemia.

Wed Jul 29 00:00:00 UTC 2020

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.

The EMBO journal

Mateju D,Franzmann TM,Patel A,Kopach A,Boczek EE,Maharana S,Lee HO,Carra S,Hyman AA,Alberti S

PA529455 was used in immunocytochemistry to characterize the components of stress granules

Wed Jun 14 00:00:00 UTC 2017

生物信息学

蛋白别名: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

基因别名: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

功能

DNA binding mRNA binding ATP-dependent DNA helicase activity ATP-dependent RNA helicase activity endonuclease activity protein binding ATP binding poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding helicase activity nuclease activity hydrolase activity RNA metabolism protein nucleic acid metabolism protein

参与通路

transport Ras protein signal transduction DNA duplex unwinding negative regulation of canonical Wnt signaling pathway nucleic acid phosphodiester bond hydrolysis

G3BP1 Polyclonal Antibody

G3BP1 Antibody (PA5-29455) in ICC/IF

产品信息

PA5-29455

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

G3BP1 Polyclonal Antibody

G3BP1 Antibody (PA5-29455) in ICC/IF

产品信息

PA5-29455

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID