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Application
FIGURE: 1 / 1
GABRA5 Antibody (PA5-97293) in WB
Western blot analysis of GABRA5 in extracts of various cell lines. Samples were incubated with GABRA5 Polyclonal antibody (Product # PA5-97293) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-97293
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 32-259 of human GABRA5 (NP_0008011)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809095
Product Specific Information
Immunogen sequence: QMPTSSVKDE TNDNITIFTR ILDGLLDGYD NRLRPGLGER ITQVRTDIYV TSFGPVSDTE MEYTIDVFFR QSWKDERLRF KGPMQRLPLN NLLASKIWTP DTFFHNGKKS IAHNMTTPNK LLRLEDDGTL LYTMRLTISA ECPMQLEDFP MDAHACPLKF GSYAYPNSEV VYVWTNGSTK SVVVAEDGSR LNQYHLMGQT VGTENISTST GEYTIMTAHF HLKRKIGY; Positive Samples: Mouse brain, Mouse skeletal muscle, Mouse pancreas, Rat brain, Rat skeletal muscle; Cellular Location: Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Target Information
GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor; GABA(A) receptor subunit alpha-5; GABA-A receptor alpha-5 subunit; GABAA receptor alpha5 subunit; GABAAR subunit alpha-5; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5; gamma-aminobutyric acid A receptor, alpha 5; Gamma-aminobutyric acid receptor subunit alpha-5; ligand-gated ion channel subunit
Gene Aliases: A230018I05Rik; Gabra-5; Gabra5
UniProt ID: (Rat) P19969, (Mouse) Q8BHJ7
Entrez Gene ID: (Rat) 29707, (Mouse) 110886
behavioral fear response
gamma-aminobutyric acid signaling pathway
chemical synaptic transmission
brain development
sensory perception of sound
associative learning
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
neuron development
inner ear receptor cell development
innervation
cochlea development
chloride transmembrane transport
positive regulation of defense response to virus by host
transport
ion transport
chloride transport
signal transduction
mitophagy in response to mitochondrial depolarization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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