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Invitrogen
GAD65 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 9
GAD65 Antibody (PA5-21297) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GAD65 was performed in Cultured Rat E18 primary cortical neuron, DIV 8. Cells fixed in 4% paraformaldehyde at RT for 15 min. Red: GAD65 Polyclonal Antibody (Product # PA5-21297) diluted at 1:250. Blue: Fluoroshield with DAPI.
产品信息
PA5-21297
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 465 and 558 of GAD65 (Uniprot ID#Q05329)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11154005
产品详细信息
Recommended positive controls: Mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2
基因别名: 6330404F12Rik; GAD(65); Gad-2; GAD2; GAD65
UniProt ID: (Human) Q05329, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Human) 2572, (Mouse) 14417, (Rat) 24380
