Invitrogen

GAD65 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3个已发表图片

7 篇参考文献

查看其他33个GAD65抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

GAD65 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

GAD65 Antibody (PA5-22260) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of GAD65 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD65 Polyclonal Antibody (Product # PA5-22260) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

GAD65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GAD65 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GAD65 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of GAD65 was observed in Mouse brain, Rat brain in comparison to Mouse kidney and Rat kidney using Anti-GAD65 polyclonal antibody (Product # PA5-22260) in Western Blot. (https://doi.org/10.1073/pnas.95.17.10055). {RE}

产品信息

PA5-22260

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 353 and 585 of GAD65 (Uniprot ID#Q05329)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11154107

产品详细信息

Recommended positive controls: mouse brain, rat brain.

Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (86%), Dog (96%), Pig (95%), Rhesus Monkey (100%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ednrb mice with hirschsprung disease are missing Gad2-expressing enteric neurons in the ganglionated small intestine.

Frontiers in cell and developmental biology

Bhave S,Guyer RA,Picard N,Omer M,Hotta R,Goldstein AM

PA5-22260 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show for the first time that ganglionated regions of the Hirschsprung gut lack a neuronal subpopulation, which may explain the persistent gastrointestinal dysfunction after surgical correction of Hirschsprung disease.

Tue Nov 01 00:00:00 EDT 2022

Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depression.

The Journal of physiology

Huang R,Worrell J,Garner E,Wang S,Homsey T,Xu B,Galer EL,Zhou Y,Tavakol S,Daneshvar M,Le T,Vinters HV,Salamon N,McArthur DL,Nuwer MR,Wu I,Leiter JC,Lu DC

PA5-22260 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that continuous cervical EES restored a cyclic breathing pattern (eupnoea) in the OFF-State, suggesting that cervical EES reversed the opioid-induced respiratory depression.

Wed Jun 01 00:00:00 EDT 2022

Lithium treatment reverses irradiation-induced changes in rodent neural progenitors and rescues cognition.

Molecular psychiatry

Zanni G,Goto S,Fragopoulou AF,Gaudenzi G,Naidoo V,Di Martino E,Levy G,Dominguez CA,Dethlefsen O,Cedazo-Minguez A,Merino-Serrais P,Stamatakis A,Hermanson O,Blomgren K

PA5-22260 was used in Western Blot to show that lithium treatment reverses irradiation-induced loss of hippocampal neurogenesis and cognitive impairment even when introduced long after the injury.

Fri Jan 01 00:00:00 EST 2021

Lithium treatment reverses irradiation-induced changes in rodent neural progenitors and rescues cognition.

Molecular psychiatry

Zanni G,Goto S,Fragopoulou AF,Gaudenzi G,Naidoo V,Di Martino E,Levy G,Dominguez CA,Dethlefsen O,Cedazo-Minguez A,Merino-Serrais P,Stamatakis A,Hermanson O,Blomgren K

PA5-22260 was used in Western Blot to show that lithium treatment reverses irradiation-induced loss of hippocampal neurogenesis and cognitive impairment even when introduced long after the injury.

Fri Jan 01 00:00:00 EST 2021

Input-specific modulation of murine nucleus accumbens differentially regulates hedonic feeding.

Nature communications

Christoffel DJ,Walsh JJ,Heifets BD,Hoerbelt P,Neuner S,Sun G,Ravikumar VK,Wu H,Halpern CH,Malenka RC

PA5-22260 was used in Immunohistochemistry to show the modulation of prefrontal cortex and anterior paraventricular thalamus excitatory inputs has opposing effects on high fat intake.

Fri Apr 09 00:00:00 EDT 2021

Increased Frataxin Expression Induced in Friedreich Ataxia Cells by Platinum TALE-VP64s or Platinum TALE-SunTag.

Molecular therapy. Nucleic acids

Cherif K,Gérard C,Rousseau J,Ouellet DL,Chapdelaine P,Tremblay JP

PA5-22260 was used in Western Blotting to show the aconitase activity was positively modulated by the frataxin level in mitochondria, and it was, thus, increased in vitro and in vivo by the increased frataxin expression.

Fri Sep 07 00:00:00 EDT 2018

GABAergic signaling linked to autophagy enhances host protection against intracellular bacterial infections.

Nature communications

Kim JK,Kim YS,Lee HM,Jin HS,Neupane C,Kim S,Lee SH,Min JJ,Sasai M,Jeong JH,Choe SK,Kim JM,Yamamoto M,Choy HE,Park JB,Jo EK

Published figure using GAD65 polyclonal antibody (Product # PA5-22260) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Anatomical and Electrophysiological Clustering of Superficial Medial Entorhinal Cortex Interneurons.

eNeuro

Martínez JJ,Rahsepar B,White JA

Published figure using GAD65 polyclonal antibody (Product # PA5-22260) in Immunohistochemistry

Thu Jun 14 00:00:00 EDT 2018

生物信息学

蛋白别名: 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2

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基因别名: 6330404F12Rik; GAD(65); Gad-2; GAD2; GAD65

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UniProt ID: (Human) Q05329, (Mouse) P48320, (Rat) Q05683

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Entrez Gene ID: (Human) 2572, (Mouse) 14417, (Rat) 24380

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功能

glutamate decarboxylase activity protein binding glutamate binding pyridoxal phosphate binding protein heterodimerization activity catalytic activity lyase activity carboxy-lyase activity decarboxylase

参与通路

glutamate decarboxylation to succinate chemical synaptic transmission neurotransmitter secretion neurotransmitter biosynthetic process response to drug carboxylic acid metabolic process