Invitrogen

GAP43 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

GAP43 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

GAP43 Antibody (PA5-34943) in ICC/IF

Immunofluorescence analysis of GAP43 was performed using SH-SY5Y differentiated neuronal and undifferentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GAP43 Polyclonal Antibody (Product # PA5-34943) at 1:200 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More

GAP43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GAP43 Antibody in Immunohistochemistry (Frozen) (IHC (F))

GAP43 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GAP43 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of GAP43 was observed in SH-SY5Y differentiated to neurons as compared to undifferentiated SH-SY5Ycells, A549, A-431 cells and Mouse lung tissue which are reported to be low to negative for GAP43 expression using Anti-GAP43 Polyclonal Antibody (Product # PA5-34943) in Western Blot. {RE}

产品信息

PA5-34943

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:30,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide contains a sequence corresponding to a region within amino acids 166 and 256 of GAP43

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2552292

产品详细信息

Recommended positive controls: SK-N-SH, IMR32, SK-N-AS, mouse fetal brain, mouse hippocampus, rat hippocampus, mouse brain, rat brain.

Predicted reactivity: Mouse (100%), Rat (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lectin histochemistry of the olfactory mucosa of Korean native cattle, Bos taurus coreanae.

Journal of veterinary science

Jang S,Kim B,Lee J,Kang S,Kim JS,Kim JC,Kim SH,Shin T,Moon C

PA5-34943 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to suggest that glycoconjugates may play a role in olfactory chemoreception, and that their labeling properties may be closely related to OM development and maturity.

Tue Nov 01 00:00:00 EDT 2022

Lectin histochemistry of the olfactory mucosa of Korean native cattle, Bos taurus coreanae.

Journal of veterinary science

Jang S,Kim B,Lee J,Kang S,Kim JS,Kim JC,Kim SH,Shin T,Moon C

Tue Nov 01 00:00:00 EDT 2022

Early Developmental PMCA2b Expression Protects From Ketamine-Induced Apoptosis and GABA Impairments in Differentiating Hippocampal Progenitor Cells.

Frontiers in cellular neuroscience

Lisek M,Mackiewicz J,Sobolczyk M,Ferenc B,Guo F,Zylinska L,Boczek T

Published figure using GAP43 polyclonal antibody (Product # PA5-34943) in Western Blot

Sat Jul 16 00:00:00 EDT 2022

生物信息学

蛋白别名: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; neuron protein F1; pp46; Protein F1

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基因别名: B-50; Basp2; GAP-43; GAP43; PP46

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UniProt ID: (Human) P17677, (Rat) P07936, (Mouse) P06837

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Entrez Gene ID: (Human) 2596, (Rat) 29423, (Mouse) 14432

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功能

phosphatidylserine binding protein binding calmodulin binding lysophosphatidic acid binding phosphatidylinositol phosphate binding

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