Invitrogen

GATA1 Polyclonal Antibody

查看其他18个GATA1抗体

抗体检测数据 (9)

GATA1 Antibody in Immunocytochemistry (ICC/IF)

GATA1 Antibody in Immunocytochemistry (ICC/IF)

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GATA1 Antibody (PA1-099) in ICC/IF

Immunofluorescent analysis of GATA1 (green) showing staining in U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA1 polyclonal antibody (Product # PA1-099) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antib... View More

GATA1 Antibody in Immunocytochemistry (ICC/IF)

GATA1 Antibody in Immunocytochemistry (ICC/IF)

GATA1 Antibody in Immunocytochemistry (ICC/IF)

GATA1 Antibody in Immunocytochemistry (ICC/IF)

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GATA1 Antibody in Western Blot (WB)

GATA1 Antibody in Western Blot (WB)

Product Image Gallery

GATA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA1 (green) showing staining in U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA1 polyclonal antibody (Product # PA1-099) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

GATA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA1 (green) showing staining in A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA1 polyclonal antibody (Product # PA1-099) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

GATA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA1 (green) showing staining in Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA1 polyclonal antibody (Product # PA1-099) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

GATA1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GATA1 (green) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA1 (Product # PA1-099) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA1 polyclonal antibody (Product # PA1-099) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA1 polyclonal antibody (Product # PA1-099) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GATA1 showing staining in the nucleus of paraffin-embedded human brain tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GATA1 polyclonal antibody (Product # PA1-099) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GATA1 Antibody in Western Blot (WB) — Western blot analysis of GATA1 was performed by loading 100 ng of GST-tagged human GATA1 and GATA2 recombinant proteins and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288) and blocked with SuperBlock T20 blocking buffer (Product # 37536) in TBST for 1 hour at room temperature. GATA1-GST fusion ptrotein was detected at ~70 kD using an GATA1 rabbit polyclonal antibody (Product # PA1-099) (left panel), stripped, and reprobed with GATA1 and GATA2 rabbit polyclonal antibodies (middle panel). Blots were stripped again and reprobed with a GST-tag (right panel) polyclonal antibody. All antibodies were used at a dilution of 1:1000 in blocking buffer overnight at 4°C or for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31460) at a dilution of 1:20,000 for 1 hour at room temperature. Recombinant GST-fusion protein breakdown bands are indicated in the right panel. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).

GATA1 Antibody in Western Blot (WB) — Western blot analysis of GATA1 was performed by loading 25 µg of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing GATA1 (Product # PA1-099) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

产品信息

PA1-099

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

N-terminal truncated human recombinant protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539869

产品详细信息

PA1-099 specifically detects GATA1 and does not cross-react with GATA2 or GATA3.

靶标信息

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Eryf1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA-binding protein 1; globin transcription factor 1; HGNC:4170; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1

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基因别名: ERYF1; GATA-1; GATA1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT

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UniProt ID: (Human) P15976, (Mouse) P17679

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Entrez Gene ID: (Human) 2623, (Mouse) 14460

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transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding DNA binding, bending chromatin DNA binding sequence-specific DNA binding C2H2 zinc finger domain binding RNA polymerase II transcription factor activity, sequence-specific DNA binding chromatin binding metal ion binding DNA-binding transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription from RNA polymerase II promoter cell-cell signaling heart development blood coagulation negative regulation of cell proliferation male gonad development organ morphogenesis tissue development regulation of glycoprotein biosynthetic process regulation of definitive erythrocyte differentiation regulation of primitive erythrocyte differentiation erythrocyte differentiation megakaryocyte differentiation platelet formation basophil differentiation eosinophil differentiation negative regulation of bone mineralization positive regulation of osteoblast proliferation embryonic hemopoiesis eosinophil fate commitment negative regulation of apoptotic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development erythrocyte development homeostasis of number of cells within a tissue positive regulation of peptidyl-tyrosine phosphorylation platelet aggregation transcriptional activation by promoter-enhancer looping dendritic cell differentiation cellular response to thyroid hormone stimulus negative regulation of transcription regulatory region DNA binding negative regulation of extrinsic apoptotic signaling pathway in absence of ligand transcription, DNA-templated regulation of transcription, DNA-templated myeloid cell differentiation cell development