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GCN1L1 Antibody (PA5-88965) in WB
Western blot analysis of GCN1L1 in extracts from wild type (WT) and GCN1L1 knockout (KO) 293T(KO) cells. Samples were incubated with GCN1L1 Polyclonal antibody (Product # PA5-88965) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-88965
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_0068271)
偶联物
形式
Liquid
浓度
1.66 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805260
产品详细信息
Immunogen sequence: LSAVLQQCLL ADVSGIDWMV RHGRSLALSV AVNVAPGRLC AGRYSSDVQE MILSSATADR IPIAVSGVRG MGFLMRHHIE TGGGQLPAKL SSLFVKCLQN PSSDIRLVAE KMIWWANKDP LPPLDPQAIK PILKALLDNT KDKNTVVRAY SDQAIVNLLK MRQGEEVFQS LSKILDVASL EVLNEVNRRS LKKLASQADS TEQVDDTILT; Positive Samples: OVCAR3, U-87MG, HepG2, HeLa
靶标信息
Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GCN1 (general control of amino-acid synthesis 1, yeast)-like 1; GCN1 eIF-2-alpha kinase activator homolog; GCN1 eIF2 alpha kinase activator homolog; GCN1 general control of amino-acid synthesis 1-like 1; GCN1-like protein 1; General control of amino-acid synthesis 1-like protein 1; HsGCN1; peroxisome proliferator activated receptor interacting complex protein; Stalled ribosome sensor GCN1; Translational activator GCN1
基因别名: GCN1; GCN1L; GCN1L1; KIAA0219; PRIC295
UniProt ID: (Human) A8KAY1
Entrez Gene ID: (Human) 10985
translation
regulation of translation
positive regulation of kinase activity
cellular response to amino acid starvation
positive regulation of transcription from RNA polymerase II promoter in response to stress
regulation of protein kinase activity
cell-cell adhesion
cellular response to leucine starvation
