Invitrogen

GCN5 Polyclonal Antibody

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GCN5 Antibody (PA5-30041) in WB

Western blot was performed using Anti-GCN5 Polyclonal Antibody (Product # PA5-30041) and a 100kDa band corresponding to GCN5 was observed across all the tested cell lines and tissues. Nuclear enriched extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2), Ramos (Lane 3), U-2 OS (Lane 4), NIH/3T3 (Lane 5), SH-SY5Y (Lane 6), HeLa (Lane 7), HCT 116 (Lane 8), and tissue extracts (30 µg lysate) of Mouse Ovary (Lane 9), Rat Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins... View More

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产品信息

PA5-30041

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 589 and 810 of Human GCN5

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547515

产品详细信息

Recommended positive controls: IMR32, NIH-3T3, JC, BCL-1.

Predicted reactivity: Mouse (100%), Rat (100%), Chicken (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; GCN5 general control of amino acid synthesis-like 2; general control of amino acid synthesis 5-like 2; General control of amino acid synthesis protein 5-like 2; general control of amino acid synthesis, yeast homolog-like 2; General control of amino acid synthesis, yeast, homolog-like 2; general control of amino acid synthesis-like 2; hGCN5; Histone acetyltransferase GCN5; Histone acetyltransferase KAT2A; Histone glutaryltransferase KAT2A; Histone succinyltransferase KAT2A; hsGCN5; K(lysine) acetyltransferase 2A; KATA2; Lysine acetyltransferase 2A; MmGCN5; PCAF-B/GCN5; STAF97

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基因别名: 1110051E14Rik; AW212720; GCN5; GCN5L2; hGCN5; KAT2A; mmGCN5; PCAF-b

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UniProt ID: (Human) Q92830, (Mouse) Q9JHD2

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Entrez Gene ID: (Human) 2648, (Mouse) 14534

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