Invitrogen

GCN5 Monoclonal Antibody (S.775.4)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他8个GCN5抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

GCN5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

GCN5 Antibody (MA5-14884) in ICC/IF

Immunofluorescent analysis of GCN5L2 in HeLa cells using a GCN5L2 monoclonal antibody (Product # MA5-14884) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Product Image Gallery

GCN5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with GCN5 siRNA and reduction of signal was observed in Western Blot using GCN5 Monoclonal Antibody (Product # MA5-14884). {KD}

产品信息

MA5-14884

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:200-1:800

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:200

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

S.775.4

抗原

Synthetic peptide corresponding to the amino terminus of human GCN5L2

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

134 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10978625

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the related PCAF protein.

靶标信息

SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

KAT2A/E2F1 Promotes Cell Proliferation and Migration via Upregulating the Expression of UBE2C in Pan-Cancer.

Genes

Lin S,Qiu L,Liang K,Zhang H,Xian M,Chen Z,Wei J,Fu S,Gong X,Ding K,Zhang Z,Hu B,Zhang X,Duan Y,Du H

MA5-14884 was used in Immunocytochemistry-immunofluorescence to find that the expression of these three genes was significantly higher in more than 10 tumor types compared to normal tissue.

Sat Oct 08 00:00:00 EDT 2022

LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling.

Nature communications

Huang X,Pan L,Zuo Z,Li M,Zeng L,Li R,Ye Y,Zhang J,Wu G,Bai R,Zhuang L,Wei L,Zheng Y,Su J,Deng J,Deng S,Zhang S,Zhu S,Che X,Wang C,Wu C,Chen R,Lin D,Zheng J

MA5-14884 was used in Western Blot to demonstrate that LINC00842 plays a role in promoting PDAC malignancy and thus might serve as a druggable target.

Tue Jun 22 00:00:00 EDT 2021

生物信息学

蛋白别名: GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; GCN5 general control of amino acid synthesis-like 2; general control of amino acid synthesis 5-like 2; General control of amino acid synthesis protein 5-like 2; general control of amino acid synthesis, yeast homolog-like 2; General control of amino acid synthesis, yeast, homolog-like 2; general control of amino acid synthesis-like 2; hGCN5; Histone acetyltransferase GCN5; Histone acetyltransferase KAT2A; Histone glutaryltransferase KAT2A; Histone succinyltransferase KAT2A; hsGCN5; K(lysine) acetyltransferase 2A; KATA2; Lysine acetyltransferase 2A; MmGCN5; PCAF-B/GCN5; STAF97

查看更多收起

基因别名: 1110051E14Rik; AW212720; GCN5; GCN5L2; hGCN5; KAT2A; mmGCN5; PCAF-b

查看更多收起

UniProt ID: (Human) Q92830, (Mouse) Q9JHD2

查看更多收起

Entrez Gene ID: (Human) 2648, (Mouse) 14534, (Rat) 303539

查看更多收起

功能