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GFP Antibody (MA5-50340) in ICC/IF
Immunocytochemical analysis of GFP using GFP Recombinant Monoclonal Antibody (Product # MA5-50340) in Paraffin embedded mock and GFP-tagged protein transfected 293T cells at a dilution of 1:1,000. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min. Red: GFP-tagged protein stained by GFP antibody (MA5-50340) diluted at 1:1,000. Green: GFP-tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI.
产品信息
MA5-50340
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL1845
抗原
Full length GFP recombinant protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3090470
靶标信息
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
