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GLD2 Antibody (PA5-89106) in WB
Western blot analysis of GLD2 in extracts of various cell lines. Samples were incubated with GLD2 Polyclonal antibody (Product # PA5-89106) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-89106
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAPD4 (NP_7761582)
偶联物
形式
Liquid
浓度
0.88 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805359
产品详细信息
Immunogen sequence: MFPNSILGRP PFTPNHQQHN NFFTLSPTVY SHQQLIDAQF NFQNADLSRA VSLQQLTYGN VSPIQTSASP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TVVNQIVPLS GERRYSMPPL FHTHYVPDIV RCVPPFREIA FLEPREITLP EAKDKLSQQI LELFETCQQQ ISDLKKKELC RTQLQREIQL LFPQSRLFLV GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL; Positive Samples: U-87MG, BxPC-3, Mouse heart, Rat heart; Cellular Location: Cytoplasm, Nucleus
靶标信息
GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytoplasmic poly(A) polymerase; hGLD-2; mGLD-2; PAP-associated domain-containing protein 4; Poly(A) RNA polymerase GLD2; Terminal nucleotidyltransferase 2; Terminal uridylyltransferase 2; TUTase 2
基因别名: 8030446C20Rik; AI649009; GLD-2; GLD2; Palp4; PAPD4; TENT2; TUT2
UniProt ID: (Human) Q6PIY7, (Rat) Q5U315, (Mouse) Q91YI6
Entrez Gene ID: (Human) 167153, (Rat) 361878, (Mouse) 100715
