Invitrogen

GLT-1 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5个已发表图片

7 篇参考文献

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说明书

实验方案

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说明书

实验方案

技术支持

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GLT-1 Antibody (PA5-17099) in WB

Western blot was performed using Anti-GLT-1 Polyclonal Antibody, (Product # PA5-17099) and band at ~55 kDa corresponding to GLT-1 was observed in Mouse and Rat Brain and Cerebellum but not in Kidney and Lung which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Mouse Kidney (Lane 3), Mouse Lung (Lane 4), Rat Brain (Lane 5), Rat Cerebellum (Lane 6), Rat Kidney (Lane 7) and Rat Lung (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Re... View More

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GLT-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of GLT-1 was observed in Mouse and Rat Brain and Cerebellum and not in Kidney and Lung tissues using GLT-1 Polyclonal Antibody (Product # PA5-17099) in western blot. {RE}

产品信息

PA5-17099

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human EAAT2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

22 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10978571

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

mGlu3 Metabotropic Glutamate Receptors as a Target for the Treatment of Absence Epilepsy: Preclinical and Human Genetics Data.

Current neuropharmacology

Celli R,Striano P,Citraro R,Di Menna L,Cannella M,Imbriglio T,Koko M,De Sarro G,Monn JA,Battaglia G,Luijtelaar GV,Nicoletti F,Russo E,Leo A

Published figure using GLT-1 polyclonal antibody (Product # PA5-17099) in Western Blot

Fri Feb 03 00:00:00 EST 2023

Mature iPSC-derived astrocytes of an ALS/FTD patient carrying the TDP43 mutation display a mild reactive state and release polyP toxic to motoneurons.

Frontiers in cell and developmental biology

Rojas F,Aguilar R,Almeida S,Fritz E,Corvalán D,Ampuero E,Abarzúa S,Garcés P,Amaro A,Diaz I,Arredondo C,Cortes N,Sanchez M,Mercado C,Varela-Nallar L,Gao FB,Montecino M,van Zundert B

PA5-17099 was used in Immunocytochemistry to establish that human astrocytes carrying the TDP43A90V mutation exhibit a cell-autonomous pathological signature, hence providing an experimental model to decipher the molecular mechanisms underlying the generation of the neurotoxic phenotype.

Sat Mar 23 00:00:00 EDT 2024

Recurrent SLC1A2 variants cause epilepsy via a dominant negative mechanism.

Annals of neurology

Stergachis AB,Pujol-Giménez J,Gyimesi G,Fuster D,Albano G,Troxler M,Picker J,Rosenberg PA,Bergin A,Peters J,El Achkar CM,Harini C,Manzi S,Rotenberg A,Hediger MA,Rodan LH

PA5-17099 was used in Western Blotting to demonstrate that treatment of a 20-month-old SLC1A2-related epilepsy patient with the SLC1A2-modulating agent ceftriaxone did not result in a significant change in daily spasm count.

Sat Jun 01 00:00:00 EDT 2019

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2.

The Journal of biological chemistry

Simonin A,Montalbetti N,Gyimesi G,Pujol-Giménez J,Hediger MA

PA5-17099 was used in western blot to study the importance of serine 364 on glutamate transporter function

Fri Dec 18 00:00:00 EST 2015

HFE polymorphisms affect cellular glutamate regulation.

Neurobiology of aging

Mitchell RM,Lee SY,Simmons Z,Connor JR

PA5-17099 was used in western blot to examine the role of HFE polymorphisms in neurodegenerative diseases

Wed Jun 01 00:00:00 EDT 2011

Functional expression, purification and high sequence coverage mass spectrometric characterization of human excitatory amino acid transporter EAAT2.

Protein expression and purification

Ye R,Rhoderick JF,Thompson CM,Bridges RJ

PA5-17099 was used in western blot to investigate a new efficient approach to produce and characterize human excitatory amino acid transporter 2

Mon Nov 01 00:00:00 EDT 2010

生物信息学

蛋白别名: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2

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基因别名: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2

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UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596

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Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482

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功能

L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding amino acid transmembrane transporter activity glutamate:sodium symporter activity symporter activity sodium:dicarboxylate symporter activity primary active transporter

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