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GPLD1 Antibody (PA5-104013) in ICC/IF
Immunocytochemistry analysis of GPLD1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GPLD1 polyclonal antibody (Product # PA5-104013) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-104013
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human GPLD1(Accession P80108), corresponding to amino acid residues F247-S297.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853344
产品详细信息
Antibody detects endogenous levels of total GPLD1.
靶标信息
GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with polipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPLD1, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; glycosylphosphatidylinositol specific phospholipase D1, isoform 2; GPI-PLD; GPI-specific phospholipase D; MGC22590; Phosphatidylinositol-glycan-specific phospholipase D; PI-G PLD
基因别名: 6330541J12Rik; AW546131; GPIPLD; GPIPLDM; GPLD1; PIGPLD; PIGPLD1; PLD
UniProt ID: (Human) Q15128
Entrez Gene ID: (Human) 2822, (Mouse) 14756
ossification
cell migration involved in sprouting angiogenesis
chondrocyte differentiation
complement receptor mediated signaling pathway
GPI anchor release
negative regulation of cell proliferation
insulin receptor signaling pathway
response to glucose
positive regulation of endothelial cell migration
positive regulation of alkaline phosphatase activity
positive regulation of triglyceride biosynthetic process
negative regulation of triglyceride catabolic process
positive regulation of glucose metabolic process
positive regulation of high-density lipoprotein particle clearance
cellular response to insulin stimulus
cellular response to drug
hematopoietic stem cell migration
positive regulation of insulin secretion involved in cellular response to glucose stimulus
positive regulation of apoptotic process
positive regulation of cytolysis
phosphatidylcholine metabolic process
positive regulation of membrane protein ectodomain proteolysis
positive regulation of secretion
transepithelial transport
cellular response to calcium ion
cellular response to cholesterol
cellular response to triglyceride
cellular response to pH
hematopoietic stem cell migration to bone marrow
regulation of cellular response to insulin stimulus
cellular response to inorganic substance
cellular response to iron(II) ion
