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GPR143 Antibody (PA5-120094) in WB
Western blot analysis of GPR143 in extracts of various cell lines. Samples were incubated with GPR143 Polyclonal antibody (Product # PA5-120094) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
产品信息
PA5-120094
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2).
偶联物
Unconjugated
形式
Liquid
浓度
0.98 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2913666
产品详细信息
Positive test controls include: U-251MG, BT-474, SW480, Mouse liver, Mouse kidney, Rat kidney. The target is usually found in the following locations: Apical cell membrane, Golgi apparatus, Lysosome membrane, Melanosome membrane, Multi-pass membrane protein.
Immunogen sequence: TGCSLGFQSP RKEIQWESLT TSAAEGAHPS PLMPHENPAS GKVSQVGGQT SDEALSMLSE GSDASTIEIH TASESCNKNE GDPALPTHGD L
靶标信息
Ocular albinism type 1 protein is a conserved integral membrane protein with seven transmembrane domains. It is expressed in the eye and epidermal melanocytes. Defects in GPR143 are the cause of ocular albinism type 1 (OA1); also known as Nettleship-Falls type ocular albinism. OA1 is an X-linked disorder characterized by severe impairment of visual acuity, retinal hypopigmentation and the presence of macromelanosomes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: G-protein coupled receptor 143; homolog of human ocular albinism 1 (Nettleship-Falls); MOA1; ocular albinism 1; Ocular albinism type 1 protein; Ocular albinism type 1 protein homolog
基因别名: GPR143; NYS6; OA1; RGD1565799
UniProt ID: (Human) P51810, (Mouse) P70259
Entrez Gene ID: (Human) 4935, (Mouse) 18241, (Rat) 302619
eye pigment biosynthetic process
signal transduction
G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
visual perception
melanosome localization
melanosome transport
melanosome organization
calcium-mediated signaling using intracellular calcium source
phosphatidylinositol-mediated signaling
regulation of calcium-mediated signaling
regulation of melanosome transport
regulation of melanosome organization
chemical synaptic transmission
