Invitrogen

GPX1 Polyclonal Antibody

GPX1 Antibody in Immunocytochemistry (ICC/IF)

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GPX1 Antibody (PA5-26323) in ICC/IF

Immunocytochemistry analysis of GPX1 in HepG2 cells. Samples were incubated in GPX1 polyclonal antibody (Product # PA5-26323) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

GPX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-26323

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫细胞化学 (ICC/IF)

1:10-1:50

流式细胞分析 (Flow)

1:25

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.0 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2543823

靶标信息

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

NRF2/ARE mediated antioxidant response to glaucoma: role of glia and retinal ganglion cells.

Acta neuropathologica communications

Naguib S,Backstrom JR,Artis E,Ghose P,Stahl A,Hardin R,Haider AA,Ang J,Calkins DJ,Rex TS

PA5-26323 was used in Western Blot to provide neuroprotection.

Tue Oct 24 00:00:00 EDT 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

PA5-26323 was used in Western Blotting to demonstrate TCam-2 spheroid sensitivity to acute s-microgravity exposure and indicate the capability of these cells to trigger compensatory mechanisms that allow them to overcome the exposure to altered gravitational force.

Sat Aug 19 00:00:00 EDT 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Sat Aug 19 00:00:00 EDT 2023

Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients.

Journal of cachexia, sarcopenia and muscle

Mallard J,Hucteau E,Charles AL,Bender L,Baeza C,Pélissie M,Trensz P,Pflumio C,Kalish-Weindling M,Gény B,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

PA5-26323 was used in Western Blotting to investigating overall skeletal muscle mitochondrial homeostasis in early breast cancer patients undergoing chemotherapy, including mitochondrial quantity, function, and dynamics.

Wed Jun 01 00:00:00 EDT 2022

Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension.

Redox biology

Naguib S,Backstrom JR,Gil M,Calkins DJ,Rex TS

Published figure using GPX1 polyclonal antibody (Product # PA5-26323) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension.

Redox biology

Naguib S,Backstrom JR,Gil M,Calkins DJ,Rex TS

Published figure using GPX1 polyclonal antibody (Product # PA5-26323) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats.

PloS one

Migliaccio V,Scudiero R,Sica R,Lionetti L,Putti R

PA5-26323 was used in Western Blotting to aimed to evaluate hepatic oxidative stress and anti-oxidant systems response in rats exposed to HFD and/or non-toxic dose of dichlorodiphenyldichloroethylene.

Wed Jan 08 00:00:00 EST 2020

生物信息学

蛋白别名: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1

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基因别名: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx; GSHPx-1; GSHPX1

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UniProt ID: (Human) P07203, (Rat) Q91WZ5, (Mouse) P11352

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Entrez Gene ID: (Human) 2876, (Rat) 24404, (Mouse) 14775

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功能

glutathione peroxidase activity selenium binding SH3 domain binding glutathione binding phospholipid-hydroperoxide glutathione peroxidase activity peroxidase activity protein binding oxidoreductase activity peroxidase

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