Invitrogen

GRB7 Polyclonal Antibody

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抗体检测数据 (2)
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GRB7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GRB7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GRB7 Antibody (PA5-79323) in IHC (P)

Immunohistochemistry analysis of GRB7 on paraffin-embedded human Esophagus Squama cancer tissue. Sample was incubated with GRB7 polyclonal antibody (Product# PA5-79323).

GRB7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GRB7 Antibody in Western Blot (WB)

GRB7 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

GRB7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GRB7 on paraffin-embedded human Esophagus Squama cancer tissue. Sample was incubated with GRB7 polyclonal antibody (Product# PA5-79323).

GRB7 Antibody in Western Blot (WB) — Western blot analysis of GRB7 in Lane 1: rat testis tissue lysate, Lane 2: SMMC whole cell lysate, Lane 3: HeLa whole cell lysate, Lane 4: A549 whole cell lysate, Lane 5: SW620 whole cell lysate using 40 µg per well. Sample was incubated with GRB7 (Product # PA5-79323) at a dilution of 0.5 µg/mL.

GRB7 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 3 Focal adhesion-related protein complexes are major and common humoral antigens in tumor microenvironments. a GO enrichment analysis of the eight identified protein antigens. b - i Fluorescent immunocytochemistry of the identified protein antigens (green) along with focal adhesion kinase (FAK) (red) onto a human gastric cancer cell line MKN1. The blue color represents Hoechst nuclear staining. The white bars indicate 20 mum. The white arrowheads indicate representative hot spots of colocalization between protein antigens and focal adhesions (only in b - f ). Similar colocalizations were reproducibly observed in all analyzed cells with formations of visible focal adhesions (only in b - f ). The same immunocytochemical staining was also performed onto another human gastric cancer cell line GSU, obtaining similar results (Supplementary Fig. 4 ). j Co-immunoprecipitation experiments of the FAK complex. The upper and lower panels at the left side show co-IP using anti-FAK antibody for MKN1 and anti-GRB7 antibody for GSU, respectively, followed by immunoblots using the indicated antibodies. The right-side panels show co-IP using anti-FAK antibody for A549, a lung adenocarcinoma cell line, and HEK293 transfected with GRB7-myc/His construct, respectively, followed by immunoblots using the indicated antibodies. Co-IP experiments were conducted at least twice.

产品信息

PA5-79323

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

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免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

免疫细胞化学 (ICC/IF)

-

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence in the middle region of human GRB7 (124-141aa ARHVCEMLVQRAHALSDE).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2746439

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

PA5-79323 was used in Western Blot, Immunoprecipitation, Immunocytochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

PA5-79323 was used in Western Blot, Immunoprecipitation, Immunocytochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

PA5-79323 was used in Western Blot, Immunoprecipitation, Immunocytochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

PA5-79323 was used in Western Blot, Immunoprecipitation, Immunocytochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

PA5-79323 was used in Western Blot, Immunoprecipitation, Immunocytochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

生物信息学

蛋白别名: B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein; growth factor receptor binding protein GRB7; Growth factor receptor-bound protein 7; Growth factor receptor-bound protein 7 (GRB7 adapter protein) (Epidermal growth factor receptor GRB-7); OTTHUMP00000164352

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基因别名: GRB7; mKIAA4028

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UniProt ID: (Human) Q14451, (Mouse) Q03160, (Rat) Q9QZC5

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Entrez Gene ID: (Human) 2886, (Mouse) 14786, (Rat) 84427

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RNA binding SH3/SH2 adaptor activity protein binding protein kinase binding phosphatidylinositol binding identical protein binding lipid binding scaffold/adaptor protein

参与通路

epidermal growth factor receptor signaling pathway positive regulation of signal transduction negative regulation of translation positive regulation of cell migration stress granule assembly ERBB2 signaling pathway leukocyte migration signal transduction