Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
GRK2 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 5
GRK2 Antibody (PA5-77852) in WB
Western blot was performed using GRK2 Polyclonal Antibody (Product # PA5-77852) and a 80 kDa band corresponding to GRK2 was observed across cell lines and tissue extracts along with an uncharacterized band (*) at ~140 kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of HEK293 (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), THP1 (Lane 4), tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 5), Mouse Spleen (Lane 6) and Mouse Heart (Lane 7) of Fig a; and MCF7 (Lane 1), MCF10A (Lane 2), Mouse Spleen (Lane 3) and Mouse Heart (La... View More
产品信息
PA5-77852
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.212 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735885
产品详细信息
Positive Control: Jurkat, Raji, NCI-H929
Predicted Reactivity: Mouse (100%), Rat (100%), Pig (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: adrenergic beta receptor kinase 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-AR kinase-1; Beta-ARK-1; EC 2.7.1.126; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2
基因别名: Adrbk-1; ADRBK1; BARK; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK2
UniProt ID: (Mouse) Q99MK8, (Human) P25098
Entrez Gene ID: (Mouse) 110355, (Human) 156
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
G-protein coupled receptor kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Edg-2 lysophosphatidic acid receptor binding
beta-adrenergic receptor kinase activity
alpha-2A adrenergic receptor binding
non-receptor serine/threonine protein kinase
regulation of the force of heart contraction
desensitization of G-protein coupled receptor protein signaling pathway
negative regulation of the force of heart contraction by chemical signal
protein phosphorylation
response to oxidative stress
signal transduction
G-protein coupled receptor signaling pathway
tachykinin receptor signaling pathway
heart development
response to organic cyclic compound
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
viral genome replication
receptor internalization
positive regulation of catecholamine secretion
termination of G-protein coupled receptor signaling pathway
follicle-stimulating hormone signaling pathway
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of striated muscle contraction
viral entry into host cell
cardiac muscle contraction
G-protein coupled acetylcholine receptor signaling pathway
