Invitrogen

GSDMD Polyclonal Antibody

GSDMD Antibody in Immunocytochemistry (ICC/IF)

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GSDMD Antibody (PA5-104324) in ICC/IF

Immunofluorescent analysis of GSDMD in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GSDMD polyclonal antibody (Product # PA5-104324) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

GSDMD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GSDMD Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))

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产品信息

PA5-104324

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human GSDMD(Accession P57764), corresponding to amino acid residues Y158-I208.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853634

产品详细信息

Antibody detects endogenous levels of total GSDMD.

靶标信息

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。