Invitrogen

GSK3 alpha Polyclonal Antibody

查看其他17个GSK3 alpha抗体

抗体检测数据 (2)

GSK3 alpha Antibody in Immunocytochemistry (ICC/IF)

GSK3 alpha Antibody in Immunocytochemistry (ICC/IF)

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GSK3 alpha Antibody (PA5-87470) in ICC/IF

Immunofluorescence analysis of GSK3 alpha in NIH/3T3 cells. Samples were incubated with GSK3 alpha Polyclonal antibody (Product # PA5-87470) using a dilution of 1:100. Blue: DAPI for nuclear staining.

GSK3 alpha Antibody in Immunocytochemistry (ICC/IF)

GSK3 alpha Antibody in Western Blot (WB)

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GSK3 alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GSK3 alpha in NIH/3T3 cells. Samples were incubated with GSK3 alpha Polyclonal antibody (Product # PA5-87470) using a dilution of 1:100. Blue: DAPI for nuclear staining.

GSK3 alpha Antibody in Western Blot (WB) — Western blot analysis of GSK3 alpha in extracts of various cell lines. Samples were incubated with GSK3 alpha Polyclonal antibody (Product # PA5-87470) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

产品信息

PA5-87470

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:20-1:100

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3alpha (NP_0639372)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.01 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2804176

产品详细信息

Immunogen sequence: LGTQLPNNRP LPPLFNFSAG ELSIQPSLNA ILIPPHLRSP AGTTTLTPSS QALTETPTSS DWQSTDATPT LTNSS; Positive Samples: U-87MG, A-431, HeLa, B-cell, Mouse liver

靶标信息

Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638). Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp686D0638; Glycogen synthase kinase-3 alpha; GSK-3 alpha; gsk-3-alpha; OTTHUMP00000198368; Serine/threonine-protein kinase GSK3A

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基因别名: 2700086H06Rik; GSK3A

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UniProt ID: (Human) P49840, (Mouse) Q2NL51

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Entrez Gene ID: (Human) 2931, (Mouse) 606496

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protein serine/threonine kinase activity protein binding ATP binding protein kinase A catalytic subunit binding tau-protein kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

参与通路

regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis glycogen metabolic process protein phosphorylation dopamine receptor signaling pathway nervous system development insulin receptor signaling pathway negative regulation of UDP-glucose catabolic process Wnt signaling pathway positive regulation of cAMP biosynthetic process negative regulation of TOR signaling cellular response to insulin stimulus cellular response to interleukin-3 IRE1-mediated unfolded protein response proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development positive regulation of adrenergic receptor signaling pathway negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway in absence of ligand chemical synaptic transmission, postsynaptic positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitophagy positive regulation of protein targeting to mitochondrion negative regulation of glycogen synthase activity, transferring glucose-1-phosphate negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity positive regulation of glycogen (starch) synthase activity carbohydrate metabolic process regulation of gene expression by genetic imprinting negative regulation of signal transduction positive regulation of peptidyl-threonine phosphorylation phosphorylation cell migration positive regulation of peptidyl-serine phosphorylation hypermethylation of CpG island positive regulation of transcription from RNA polymerase II promoter negative regulation of transferase activity cellular response to lithium ion cellular response to organic cyclic compound