Invitrogen

GSTT1 Polyclonal Antibody

GSTT1 Antibody in Immunocytochemistry (ICC/IF)

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GSTT1 Antibody (PA5-101609) in ICC/IF

Immunocytochemistry analysis of GSTT1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GSTT1 polyclonal antibody (Product # PA5-101609) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-101609

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human GSTT1(Accession P30711), corresponding to amino acid residues M1-N49.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2851043

产品详细信息

Antibody detects endogenous levels of total GSTT1.

靶标信息

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

仅用于科研。不用于诊断过程。未经明确授权不得转售。