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Gaussia luciferase Antibody (PA1-181) in WB
Western blot analysis of secreted Gaussia luciferase was performed using 293T cells stably expressing Gaussia or Cypridina luciferase. Cells were grown overnight in serum free (B27 supplemented) DMEM with or without 10uM Brefeldin A. Equal amounts (75 µg) of approximately 10x concentrated cell culture medium from treated or untreated cells, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) were loaded onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product #... View More
产品信息
PA1-181
应用
建议稀释比
已发表文章
产品规格
种属反应
Luciferase
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified recombinant Gaussia luciferase expressed in E. coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539912
产品详细信息
Western blot analysis of PA1-181 detects a ~20 kDa protein in the supernatant of Gaussia luciferase-secreting cells.
PA1-181 can be used for activity-independent verification of Gaussia luciferase expression.
靶标信息
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. Gaussia luciferase can be measured individually or in multiplex assays, such as the Gaussia-Firefly Luciferase Dual Assay Kit (Product # 16181). The Gaussia luciferase has been codon optimized for enhanced performance.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Gaussia luciferase
