Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Goat anti-Armenian Hamster IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 6
Armenian Hamster IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A78967) in ICC/IF
Immunofluorescence analysis of Goat anti-Armenian Hamster IgG (H+L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A78967, 1:1,000 dilution) was performed on fixed and permeabilized T-47D cells stained with MUC1 Monoclonal Antibody (Product # MA5-11202, 1:100 dilution). Panel a) shows representative cells that were stained for detection and localization of MUC1 protein, Panel b) is st... View More
产品信息
A78967
应用
建议稀释比
免疫印迹 (WB)
Assay-dependent
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
1-2 µg/mL
流式细胞分析 (Flow)
1-4 µg/mL
产品规格
种属反应
Hamster
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG
交叉吸附
against Bovine, Human, Mouse, Rabbit and Rat serum proteins
抗体形式
Whole Antibody
产品详细信息
This antibody specifically detects Armenian hamster IgG and shows negligible reactivity to Syrian hamster IgG as tested by ELISA and immunofluorescence.
This goat anti-armenian hamster IgG (H+L) whole secondary antibody has been affinity-purified and shows minimum cross-reactivity to bovine, human, mouse, rabbit, rat serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondary antibodies flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g. flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Product will be shipped at Room Temperature.
靶标信息
Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
