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Invitrogen
Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Chicken IgY (H+L) Secondary Antibody (PA1-28798) in WB
Chemiluminescent western blot was performed using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP (Product # PA1-28798). Whole cell extracts of MCF 10A (Lane 1, 2, 3, 4), SH-SY5Y (Lane 5, 6, 7), HaCaT (Lane 8), HeLa (Lane 9) and MCF7 (Lane 10) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03222BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # I... View More
产品信息
PA1-28798
应用
建议稀释比
免疫印迹 (WB)
1:2,000-1:10,000
免疫组化 (IHC)
1:1,000-1:2,500
酶联免疫吸附实验 (ELISA)
1:20,000-1:100,000
产品规格
种属反应
Chicken
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Full length native chicken IgY (purified)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% gentamicin sulfate
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_10988187
靶标
IgY
抗体形式
Whole Antibody
产品详细信息
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
