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Invitrogen
F(ab')2-Goat anti-Human IgG Fc Secondary Antibody, FITC
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Human IgG Fc Secondary Antibody (H10101C) in ICC/IF
Immunofluorescence analysis of F(ab)2-Goat anti-Human IgG Fc Secondary Antibody, FITC (Product # H10101C) was performed using anti-Human MUC1 antibody (Product # MA5- 42157) with T-47D (positive) and Caco-2 (negative) cells. The cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA, then incubated with following primary antibodies at 1:100 dilution at 4 degr... View More
产品信息
H10101C
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1:250
流式细胞分析 (Flow)
Assay-Dependent
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
纯化类型
purified
保存液
PBS with BSA
内含物
0.1% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536545
靶标
IgG Fc
抗体形式
F(ab')2 Fragment
产品详细信息
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Analyte Specific Reagent
