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Invitrogen
Goat anti-Human IgG Fc Recombinant Secondary Antibody, Alexa Fluor™ 647
高级验证
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
图: 1 / 5
Human IgG Fc Secondary Antibody (A55749) in ICC/IF
Immunofluorescence analysis of Goat anti-Human IgG Fc Recombinant Secondary Antibody, Alexa Fluor™ 647 (Product # A55749, 1:500 dilution) was performed on fixed and permeabilized T-47D cells stained with MUC1/ Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, 1:500 dilution). Panel a) shows representative cells that were stained for detection and localization of MUC1 protein, Panel b... View More
产品信息
A55749
应用
建议稀释比
免疫印迹 (WB)
1:1,000
免疫细胞化学 (ICC/IF)
1:500-1:2,000
流式细胞分析 (Flow)
1:1,000
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
3H8L9
抗原
Purified Human IgG
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4
内含物
5mM sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2925774
靶标
IgG Fc
抗体形式
Whole Antibody
产品详细信息
This Goat anti-Human IgG Fc Recombinant Secondary Antibody specifically detects human IgG Fc with no cross-reactivity to human IgA or IgM or various species immunoglobulins.
The sensitivity and specificity of each lot is confirmed using ELISA.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
