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Invitrogen
Goat anti-Human IgG (Heavy chain) Secondary Antibody
产品信息
31118
应用
建议稀释比
免疫印迹 (WB)
Assay-dependent
免疫组化 (IHC)
Assay-dependent
免疫细胞化学 (ICC/IF)
Assay-dependent
酶联免疫吸附实验 (ELISA)
Assay-dependent
原位杂交 (ISH)
Assay-dependent
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.8, with 20mg/mL sucrose
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
运输条件
Ambient
RRID
AB_228261
靶标
IgG (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
Product # 31118 has been successfully used in Western blot, IF, ICC, IHC, ELISA, and ISH applications.
Product # 31118 reacts with the heavy chains of human IgG, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody has minimal cross-reactivity with human IgE and human IgM. However, this antibody may cross-react with immunoglobulins from other species.
The activity measured by a solid phase binding assay is 91%.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
