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Invitrogen
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
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Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16083) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, TRITC was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL mouse primary antibody for 3 hou... View More
产品信息
A16083
应用
建议稀释比
免疫细胞化学 (ICC/IF)
4 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
552/578 nm
查看光谱
形式
Lyophilized
浓度
2.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 1% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2534757
靶标
IgG
交叉吸附
Against bovine, goat, human, rabbit and rat IgG
抗体形式
Whole Antibody
产品详细信息
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. 1 year from date of receipt. Prepare working dilution prior to use and then discard. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, bovine, goat, human, rabbit or rat IgG.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
