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Identifyn
F(ab')2-Goat anti-Mouse IgG, (H+L) SRM Identifyn™ Secondary Antibody, Alexa Fluor™ 750
Application
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Mouse IgG, (H+L) SRM Secondary Antibody (SA000084) in ICC/IF
HeLa Cells were grown on acid-etched 1.5H cover glass to ~50% confluency, followed by -20°C methanol fixation and blocked with Thermo Fisher, Fish Serum Blocker cat#37527X3. The first primary antibody, Golgi GOLPH2 Polyclonal Antibody, was incubated overnight at 4°C at a dilution of 1:100. Cells were washed 3X in PBS. Identifyn™ 488 secondary antibody was incubated at room temperature for 1 hour at a diluti... View More
产品信息
SA000084
应用
建议稀释比
免疫印迹 (WB)
1:2,000
免疫细胞化学 (ICC/IF)
1:250-1:1,000
产品规格
种属反应
Bovine,
Horse,
Human,
Pig,
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Proprietary Immunogen.
偶联物
激发/发射光谱
752/776 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 10% glycerol
内含物
0.02% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
靶标
IgG
抗体形式
F(ab'2) Fragment
产品详细信息
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:1,000
Widefield SIM - 1:1,000
Confocal NIR - 1:1,000
Recommended dilution for Super Resolution microscopy:
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:250
Detailed protocols can be found below under Methods & Protocols.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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