Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, APC, eBioscience™
图: 1 / 1
Mouse IgG (H+L) Secondary Antibody (17-4010-82) in Flow
Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (blue histogram) or Anti-Human CD4 Purified (a mouse IgG2b isotype, OKT4, Product # 14-0048-82) (purple histogram) followed by 0.25 µg of F (ab')2 Anti-Mouse IgG APC. Cells in the lymphocyte gate were used for analysis.
产品信息
17-4010-82
应用
建议稀释比
流式细胞分析 (Flow)
0.5 µg/test
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ Ig
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2573203
靶标
IgG
抗体形式
F(ab')2 Fragment
产品详细信息
Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.
This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
