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Invitrogen
Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP
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Mouse IgG (H+L) Secondary Antibody (A28177) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti SOD2 Mouse Monoclonal Antibody (Product# MA1-106, 0.25 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A28177) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 25 ... View More
产品信息
A28177
应用
建议稀释比
免疫印迹 (WB)
1:10,000-1:200,000
酶联免疫吸附实验 (ELISA)
0.05-1 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Recombinant full-length protein
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Gravity column chromatography
保存液
PBS, HRP Stabilizer
内含物
proprietary preservative
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2536163
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
