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Mouse IgG1 Cross-Adsorbed Secondary Antibody (31862) in Flow
Flow cytometry analysis of Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, PE (Product # 31862) was performed using K-562 cells stained with alpha Tubulin Mouse Monoclonal Antibody (Product # A11126). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with alpha Tubuli... View More
产品信息
31862
应用
建议稀释比
流式细胞分析 (Flow)
1:500
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_429716
靶标
IgG1
交叉吸附
Against human, bovine and rabbit serum proteins
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31862 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31862 reacts with the heavy chains of mouse IgG1, but not with heavy chains of other mouse IgG subclasses or with light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.
Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 82.0 at 566 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
Phycoerythrin Source: Seaweed
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. RPE Amax= 490 nm, 545 nm, and 566 nm; Emax= 580 nm.
Reconstitute with 0.5 mL of distilled water (0.5 mg/mL after restoration).
Country of Origin: USA
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
