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Mouse IgG1 Secondary Antibody (SA5-10264) in ELISA
ELISA analysis was performed using Mouse IgG1 antigen (500 ng/well, 60 min). Samples were blocked for 60 min with 1% BSA, incubated with Goat anti-Mouse IgG1 (60 min), and followed by HRP Chicken anti-Goat IgG h+l (Product # SA5-10264) for 30 min. Development was done with TMB substrate (10 min) and the reaction was stopped with 0.3M H2SO4. Results were read on a microplate reader at OD450.
产品信息
SA5-10264
应用
建议稀释比
免疫印迹 (WB)
Assay-dependent
免疫组化 (IHC)
Assay-dependent
酶联免疫吸附实验 (ELISA)
Assay-dependent
免疫电泳 (IE)
Assay-dependent
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Mouse IgG1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2868312
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
This antibody will react with mouse IgG1 as determined by ELISA and IEP techniques and has less than 1% cross reactivity with mouse IgG2a, IgG2b and IgG3. This antibody may cross react with IgG from other species.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
