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Invitrogen
F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, APC
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (31984) in ICC/IF
Immunofluorescence analysis of F (ab')2-Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, APC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Rabbit primary a... View More
产品信息
31984
应用
建议稀释比
免疫组化 (IHC)
1:50 - 1:200
免疫细胞化学 (ICC/IF)
0.5 mg/mL
流式细胞分析 (Flow)
1:500
免疫沉淀 (IP)
1:50 - 1:200
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_429727
靶标
IgG
交叉吸附
Against human, mouse and rat serum proteins
抗体形式
F(ab')2 Fragment
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31984 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31984 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 73 at 650 nm for a
1% solution for only those APC molecules to which at least one molecule of active antibody is bound)
Allophycocyanin Source: Spirulina platensis
Store product protected from light at 4°C until opened. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. Do not freeze. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Allophycocyanin Amax= 650 nm; Emax= 660 nm.
Reconstitute with 0.5 mL of distilled water (0.5 mg/mL after restoration).
Country of Origin: USA
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
