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Invitrogen
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, APC
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-10931) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, APC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Rabbit primary antibody for 3 hours at r... View More
产品信息
A-10931
应用
建议稀释比
免疫细胞化学 (ICC/IF)
4 µg/mL
流式细胞分析 (Flow)
1-10 µg/mL
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2534068
靶标
IgG
交叉吸附
Against human IgG and serum, mouse IgG and serum, and bovine serum
抗体形式
Whole Antibody
产品详细信息
Allophycocyanin, Crosslinked, Goat Anti-Rabbit IgG (H+L) is prepared from crosslinked allophycocyanin (APC) and an affinity-purified goat anti-rabbit IgG antibody. Since this phycobiliprotein is optimally excited at 633 nm, it can also be excited at 594 nm; therefore, it can be used with samples simultaneously labeled with Texas Red dye or Alexa Fluor 594 dye without the need of a second excitation source. APC can be used in flow cytometry and imaging applications and is more photostable than Cy5 conjugates.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
