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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-31558) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 514 (Product # A-31558) was performed using MCF 10A (positive model) and T-47D (negative model) cells stained with Vimentin Polyclonal Antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA f... View More
产品信息
A-31558
应用
建议稀释比
免疫印迹 (WB)
1:10,000
免疫组化 (IHC)
1-10 µg/mL
免疫细胞化学 (ICC/IF)
1-10 µg/mL
流式细胞分析 (Flow)
1-10 µg/mL
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
Alexa Fluor™ 514
激发/发射光谱
518/543 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536173
靶标
IgG
交叉吸附
Against human IgG, human serum, mouse IgG and bovine serum
抗体形式
Whole Antibody
产品详细信息
The yellow-green fluorescent Alexa Fluor 514 dye is far superior to fluorescein in both brightness and photostability, and it can be detected with standard fluorescein, Oregon Green dye or Alexa Fluor 488 dye filter sets. By using instruments that collect spectral distribution information, the signal from the Alexa Fluor 514 dye can be distinguished from both the Alexa Fluor 488 and Alexa Fluor 500 dyes.
Product will be shipped at Room Temperature.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
