Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 3
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (G-21079) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and MCF7 (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product# 720326, 0.5 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, AP conjugate (Product # G-21079) at dilutions 1:500 (Fig. 1), 1:2,000 (Fig... View More
产品信息
G-21079
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:50,000
免疫组化 (IHC)
1:500-1:2,000
酶联免疫吸附实验 (ELISA)
1:500-1:2,000
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
AP
形式
Lyophilized
浓度
2 mg/mL
纯化类型
purified
保存液
50mM tris, pH 8.0, with 30mM trehalose, 0.1M NaCl
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536529
靶标
IgG
交叉吸附
Against human IgG, human serum, mouse IgG, mouse serum and bovine serum
抗体形式
Whole Antibody
产品详细信息
The specific activity of the AP conjugates is approximately 150-350 units/mg. One unit is defined as the amount of enzymatic activity required to hydrolyze 1.0 micromole of p nitrophenyl phosphate to p-nitrophenol and inorganic phosphate in 60 seconds at 21ºC, pH 10.
Reconstituted using 0.5 mL of deionized water to make a 2 mg/mL stock solution.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
