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Invitrogen
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 594
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Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32740) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 594 (Product # A32740) was performed using MCF 10A (positive model) and A-431 (negative model) cells stained with Vimentin Polyclonal Antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked wit... View More
产品信息
A32740
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1-10 µg/mL
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
590/618 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.5
内含物
0.016% Methylisothiazolone, 0.016% Bromonitrodioxane
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2762824
靶标
IgG
交叉吸附
Against human IgG, mouse IgG, rat IgG and non-immunoglobulin serum from human, mouse and rat
抗体形式
Whole Antibody
产品详细信息
To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been cross-adsorbed against IgG from human, mouse and rat. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically
Specificity: This antibody binds to whole molecule rabbit IgG and light chains on other rabbit immunoglobulins. This antibody does not bind non-immunoglobulin serum proteins from human, mouse and rat. It has been pre-adsorbed for minimal cross-reactivity with IgG from human, mouse and rat sources.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
