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H4K16ac Polyclonal Antibody

H4K16ac Antibody in Immunocytochemistry (ICC/IF)

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H4K16ac Antibody (600-401-J00) in ICC/IF

Immunofluorescence of Rabbit Anti-Histone H4 [ac Lys16] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [ac Lys16] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [ac Lys16] is nuclear and chromosomal. Staining: Histone H4 [ac Lys16] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

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产品信息

600-401-J00

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

1:100

免疫细胞化学 (ICC/IF)

1:100

点杂交 (DB)

1:1,000

产品规格

种属反应

C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Histone H4 [ac Lys16] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 16 of human Histone H4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Histone H4 [ac Lys16] reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: acetyl Histone H4; Acetyl-H4 K16; Acetyl-H4 Lys16; Acetyl-Histone H4 K16; Acetyl-Histone H4 Lys16; CG3379-PA; CG3379-PB; CG3379-PC; CG3379-PD; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K16ac; H4K16; H4K16a; H4Lys16ac; His4r-PA; His4r-PB; His4r-PC; His4r-PD; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 1, H4l; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1, H4-IV, germinal H4-like 2 (similar to human histone cluster 1, class H4 genes); Histone Cluster 4; histone family member; Histone H2B 2; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K16; Histone H4K16ac; histone IV, family 2; RH52884p

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基因别名: BcDNA:RH52884; CELE_T10C6.11; CG31611; CG3379; CG33869; CG33871; CG33873; CG33875; CG33877; CG33879; CG33881; CG33883; CG33885; CG33887; CG33889; CG33891; CG33893; CG33895; CG33897; CG33899; CG33901; CG33903; CG33905; CG33907; CG33909; dJ160A22.2; Dmel\CG3379; Dmel_CG3379; F07B7.11; F07B7.4; F45F2.12; FBtr0082962; FO108; H4; H4-12; H4-16; H4-53; H4-I; H4-II; H4-III; H4-IV; H4-V; H4-VI; H4-VII; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; H4r; his-20; his-22; his-4; his-52; his-54; his-8; His4; His4-88CD; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; His4R; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; K06C4.12; K06C4.4; T10C6.11; X04652; XELAEV_18001418mg