Invitrogen

HEBP1 Polyclonal Antibody

HEBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HEBP1 Antibody (PA5-30609) in IHC (P)

Immunohistochemistry (Paraffin) analysis of HEBP1 was performed in paraffin-embedded mouse intestine tissue using HEBP1 Polyclonal Antibody (Product # PA5-30609) at a dilution of 1:500.

HEBP1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

HEBP1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HEBP1 Antibody — Figure 8. Neuronal cell death occurs by triggering mitochondrial-dependent apoptotic pathway in Hebp1-expressing neurons. Western blot analyses of (A and B) CytC and Mic60 leakages and (C) caspase 9 activation in Hebp1-deficient, wildtype and control neurons. Wildtype and control neurons exhibited high levels of activated caspase 9 concomitant with mitochondrial release of CytC and Mic60 into the cytosol (S). Hebp1 release was also coupled with leakage of CytC and Mic60 in these cells. In contrast, Hebp1-deficient neurons displayed no apparent activation of caspase 9 despite leakages of CytC and Mic60 from neuronal mitochondria (Mt). (D) Wildtype, control and Hebp1-deficient neurons were treated with 10 µM hemin for 24 hr. Apoptotic cells were visualized by fluorescence staining corresponding to caspase 3/7 activation (see Materials and methods). Hebp1-deficient neurons demonstrated resistance to apoptosis upon heme overload, whereas wildtype and control neurons exhibited high levels of caspase 3/7 activity. (E) Quantification of the data represented by the images shown in (D). All bar charts represent mean ± SEM. Statistical significance in the datasets was assessed by one-way ANOVA followed by Student’s t-test comparison for individual pairs of samples: ****p<0.001. All data shown are representative of results obtained from three independent experiments. {KO}

产品信息

PA5-30609

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 189 of Human HEBP1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.63 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2548083

产品详细信息

Recommended positive controls: A549, HeLa, HepG2, HCT116.

Predicted reactivity: Mouse (86%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。