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HGS Antibody (PA5-104477) in ICC/IF
Immunofluorescent analysis of HGS in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and HGS polyclonal antibody (Product # PA5-104477) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-104477
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human HGS(Accession O14964), corresponding to amino acid residues S310-Q360.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853778
产品详细信息
Antibody detects endogenous levels of total HGS.
靶标信息
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Hepatocyte growth factor-regulated tyrosine kinase substrate; HGF-regulated tyrosine kinase substrate; HGNC:4897; Hrs; human growth factor-regulated tyrosine kinase substrate; Protein pp110; SNAP-25-interacting protein Hrs-2
基因别名: Hgr; HGS; HRS; Hrs2; ZFYVE8
UniProt ID: (Human) O14964, (Rat) Q9JJ50, (Mouse) Q99LI8
Entrez Gene ID: (Human) 9146, (Rat) 56084, (Mouse) 15239
protein targeting to lysosome
autophagy
signal transduction
negative regulation of cell proliferation
membrane invagination
positive regulation of gene expression
endosomal transport
multivesicular body assembly
negative regulation of epidermal growth factor receptor signaling pathway
regulation of protein catabolic process
regulation of MAP kinase activity
negative regulation of JAK-STAT cascade
protein localization to membrane
positive regulation of exosomal secretion
endosome to lysosome transport
transport
intracellular protein transport
protein transport
