Invitrogen

HIC1 Polyclonal Antibody

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HIC1 Antibody (PA5-97235) in WB

Western blot analysis of HIC1 in extracts of various cell lines. Samples were incubated with HIC1 Polyclonal antibody (Product # PA5-97235) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-97235

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_0010916721)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.34 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2809037

产品详细信息

Immunogen sequence: AATPVIQACY PSPVGPPPPP AAEPPSGPEA AVNTHCAELY ASGPGPAAAL CASERRCSPL CGLDLSKKSP PGSAAPERPL AERELPPRPD SPPSAGPAAY KEPPLALPSL PPLPFQKLEE AAPPSDPFRG GSGSPGPEPP GRPDGPSLLY RWMKHEPGLG SYGDELGRER GSPSERCEER GGDAAVSPGG PPLGLAPPPR YPGSLDGPGA GGDGDDYKSS SEETGSSEDP SPPGGHLEGY PCPHLAYGEP E; Positive Samples: H460, HT-29, DU145, Rat heart; Cellular Location: Nucleus

靶标信息

Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and the expression of HIC-1 is also frequently suppressed in leukemia and various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad complex POZ (or BTB) domain. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。