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Invitrogen
HK2 Monoclonal Antibody (H.738.7)
高级验证
This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.
图: 1 / 6
HK2 Antibody (MA5-14849) in WB
Knockout of HK2 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1034965_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of HK2 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa HK2 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More
产品信息
MA5-14849
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
H.738.7
抗原
Synthetic peptide corresponding to residues surrounding Ser340 of sequence of human hexokinase II.
偶联物
形式
Liquid
浓度
24 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10987229
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738
基因别名: AI642394; HK2; HKII; HXK2
UniProt ID: (Human) P52789, (Mouse) O08528, (Rat) P27881
Entrez Gene ID: (Human) 3099, (Mouse) 15277, (Rat) 25059
cellular glucose homeostasis
response to ischemia
glycolytic process
lactation
apoptotic mitochondrial changes
glucose transport
negative regulation of mitochondrial membrane permeability
regulation of glucose import
carbohydrate phosphorylation
glucose 6-phosphate metabolic process
canonical glycolysis
establishment of protein localization to mitochondrion
maintenance of protein location in mitochondrion
positive regulation of mitophagy in response to mitochondrial depolarization
negative regulation of reactive oxygen species metabolic process
carbohydrate metabolic process
glucose metabolic process
metabolic process
phosphorylation
glucose catabolic process
