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HLA-DPA1 Antibody (PA5-95915) in WB
Western blot analysis of HLA-DPA1 in extracts of Raji cells. Samples were incubated with HLA-DPA1 Polyclonal antibody (Product # PA5-95915) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
产品信息
PA5-95915
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 40-220 of human HLA-DPA1 (NP_2910322)
偶联物
形式
Liquid
浓度
1.12 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2807717
产品详细信息
Immunogen sequence: YAAFVQTHRP TGEFMFEFDE DEMFYVDLDK KETVWHLEEF GQAFSFEAQG GLANIAILNN NLNTLIQRSN HTQATNDPPE VTVFPKEPVE LGQPNTLICH IDKFFPPVLN VTWLCNGELV TEGVAESLFL PRTDYSFHKF HYLTFVPSAE DFYDCRVEHW GLDQPLLKHW EAQEPIQMPE T; Positive Samples: Raji; Cellular Location: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane
靶标信息
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DP(W3); DP(W4); HLA class II histocompatibility antigen, DP alpha 1 chain; HLA-SB alpha chain; MHC class II antigen; MHC class II DP3-alpha; MHC class II DPA1; MHC class II HLA-DPA1 antigen; Primed lymphocyte test-1
基因别名: DP(W3); DP(W4); HLA-DP1A; HLA-DPA1; HLADP; HLASB; PLT1
UniProt ID: (Human) P20036
Entrez Gene ID: (Human) 3113
immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of T cell proliferation
T cell receptor signaling pathway
positive regulation of T cell activation
interferon-gamma-mediated signaling pathway
cellular response to interferon-gamma
