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HO-1 Antibody (PA5-77834) in IHC
Immunohistochemistry analysis of HMOX1 in rat brain using 10 µg of lysate. Samples were incubated with HMOX1 polyclonal antibody (Product # PA5-77834) using a dilution of 1:1000 and followed by Rabbit Anti-HO-1 at a dilution of 1:1000. Images are shown as follows: Lane 1: Rat Brain lysate, Lane 2: Purified HO-1, Lane 3: Molecular Weight Markers.
产品信息
PA5-77834
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Rat native full-length HO-1 purified from liver tissue
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735913
产品详细信息
5 µg/mL of PA5-77834 was sufficient for detection of HO-1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |Detects approximately 33kDa.
靶标信息
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein
基因别名: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32
UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901
Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368
angiogenesis
endothelial cell proliferation
wound healing involved in inflammatory response
negative regulation of leukocyte migration
heme oxidation
cellular iron ion homeostasis
response to oxidative stress
small GTPase mediated signal transduction
excretion
regulation of blood pressure
cell death
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of muscle cell apoptotic process
smooth muscle hyperplasia
cellular response to nutrient
negative regulation of mast cell cytokine production
erythrocyte homeostasis
low-density lipoprotein particle clearance
regulation of transcription from RNA polymerase II promoter in response to iron
cellular response to heat
response to nicotine
intracellular signal transduction
heme catabolic process
response to hydrogen peroxide
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of mast cell degranulation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of neuron apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
response to estrogen
positive regulation of chemokine biosynthetic process
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of vasodilation
positive regulation of smooth muscle cell proliferation
negative regulation of smooth muscle cell proliferation
regulation of sequence-specific DNA binding transcription factor activity
protein homooligomerization
iron ion homeostasis
cellular response to arsenic-containing substance
cellular response to cadmium ion
cellular response to hypoxia
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
response to hypoxia
phospholipid metabolic process
negative regulation of cell proliferation
heme metabolic process
apoptotic process
oxidation-reduction process
