Invitrogen

Histone H1 Polyclonal Antibody

Histone H1 Antibody in Immunocytochemistry (ICC/IF)

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Histone H1 Antibody (PA5-30055) in ICC/IF

Immunofluorescence analysis of Histone H1 was performed using 70% confluent log phase U-2OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H1 Rabbit Polyclonal Antibody (Product # PA5-30055) at 5 microgram/mL concentration in 0.1% BSA, incuba... View More

Histone H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Histone H1 Antibody in Western Blot (WB)

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产品信息

PA5-30055

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:20,000

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 194 of Human Histone H1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.53 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547529

产品详细信息

Recommended positive controls: HepG2, HepG2 nuclear extract, mouse liver.

Predicted reactivity: Mouse (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes.

Nature communications

Djakovic L,Hennig T,Reinisch K,Milić A,Whisnant AW,Wolf K,Weiß E,Haas T,Grothey A,Jürges CS,Kluge M,Wolf E,Erhard F,Friedel CC,Dölken L

PA5-30055 was used in Immunoprecipitation to propose a model in which allosteric changes in Pol II composition downstream of genes and ICP22-mediated interference with FACT activity explain the differential impairment of histone repositioning downstream of genes in the wake of Pol II in HSV-1 infection.

Mon Jul 31 00:00:00 EDT 2023

The 20S proteasome activator PA28γ controls the compaction of chromatin.

Journal of cell science

Fesquet D,Llères D,Grimaud C,Viganò C,Méchali F,Boulon S,Coux O,Bonne-Andrea C,Baldin V

PA5-30055 was used in Western Blot to show that PA28γ controls chromatin compaction.

Mon Feb 01 00:00:00 EST 2021

A peptide of the N terminus of GRK5 attenuates pressure-overload hypertrophy and heart failure.

Science signaling

Coleman RC,Eguchi A,Lieu M,Roy R,Barr EW,Ibetti J,Lucchese AM,Peluzzo AM,Gresham K,Chuprun JK,Koch WJ

PA5-30055 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to support a role for GRK5nt as an inhibitor of pathological GRK5 signaling that prevents heart failure.

Tue Mar 30 00:00:00 EDT 2021

Cx43 and AKAP95 regulate G1/S conversion by competitively binding to cyclin E1/E2 in lung cancer cells.

Thoracic cancer

Chen R,Chen Y,Yuan Y,Zou X,Sun Q,Lin H,Chen X,Liu M,Deng Z,Yao Y,Guo D,Zhang Y

PA5-30055 was used in western blotting to determine how the competitive binding of Cx43 and AKAP95 to cyclin E1/E2 affects G1/S conversion in lung cancer cells.

Mon Jun 01 00:00:00 EDT 2020

Linking epigenetic dysregulation, mitochondrial impairment, and metabolic dysfunction in SBMA motor neurons.

JCI insight

Pourshafie N,Masati E,Bunker E,Nickolls AR,Thepmankorn P,Johnson K,Feng X,Ekins T,Grunseich C,Fischbeck KH

PA5-30055 was used in Western Blotting to show a molecular link between altered epigenetic regulation and mitochondrial metabolism that contributes to neurodegeneration.

Thu Jul 09 00:00:00 EDT 2020

A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.

Biochemistry

Osunsade A,Prescott NA,Hebert JM,Ray DM,Jmeian Y,Lorenz IC,David Y

PA5-30055 was used in Western blot to present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant.

Tue Jan 22 00:00:00 EST 2019

生物信息学

蛋白别名: H1 antibody; H1 histone family, member 0; H1 histone family, member 1; H1 histone family, member 2; H1 histone family, member 5; H1 VAR.1; H1 VAR.3; H1 VAR.5; H1(0); H1-0; H1-0 histone; H1.0; H1.0, H1(0), H1-0; H10; H1a; H1b; H1c; H1F3; H1FV; HIST1H1B antibody; HIST1H1D; Histone 1 antibody; histone 1, family 5; histone 1, H1a; histone 1, H1b; histone 1, H1c; histone H1; Histone H1'; Histone H1(0); Histone H1-0; Histone H1.0; Histone H1.1; Histone H1.2; Histone H1.5; Histone H1a; Histone H1b; Histone H1c; Histone H1d; Histone H1s-1; Histone H1s-3; MyD196

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基因别名: 0610008C09Rik; D130017D06Rik; H1; H1(0); H1-0; H1-1; H1-2; H1-5; H1.1; H1.2; H1.5; H10; H1A; H1B; H1C; H1F0; H1F1; H1F2; H1F5; H1FV; H1s-1; H1s-3; H1var1; H1var3; HIf5; His1a; HIST1; HIST1H1A; HIST1H1B; HIST1H1C