Invitrogen

Histone H2A Recombinant Rabbit Monoclonal Antibody (15H4L7)

Histone H2A Antibody in Western Blot (WB)

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Histone H2A Antibody (700158) in WB

Western blot analysis of Histone 2A in HeLa (lane 1) NIH-3T3 (lane 2) and COS7 cell lysate (lane 3) (30 µg/each lane) using a Histone 2A recombinant rabbit monoclonal antibody (Product # 700158) at a dilution of 1 µg/mL. Samples were blocked in 5% milk/TBST and detection was performed using an AP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:10,000. NBT/BCIP was used as the substrate (Product # WB7105).

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产品信息

700158

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-2 µg/mL

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产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

15H4L7

抗原

A peptide corresponding to amino acids 2-19 of human Histone 2A.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532288

产品详细信息

This antibody is predicted to react with mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An Adversarial DNA N⁶-Methyladenine-Sensor Network Preserves Polycomb Silencing.

Molecular cell

Kweon SM,Chen Y,Moon E,Kvederaviciutė K,Klimasauskas S,Feldman DE

700158 was used in Western Blotting to show that 6mA deposition and removal are catalyzed by the Mettl4 methyltransferase and Alkbh4 dioxygenase, respectively, and that 6mA accumulation in genic elements corresponds with transcriptional silencing.

Thu Jun 20 00:00:00 EDT 2019

生物信息学

蛋白别名: Acetyl-Histone H2; H2A 615 antibody; H2A GL101 antibody; H2A histone family member; H2A histone family, member A; H2A histone family, member C; H2A histone family, member E; H2A histone family, member G; H2A histone family, member M; H2A histone family, member P; H2A histone family, member R; H2A histone family, member X; H2A histone family, member Z; H2A-clustered histone 1; H2A-clustered histone 12; H2A-clustered histone 6; H2A.1; H2A.1 antibody; H2A/d; H2a/x; H2A/z; h2ad; H2ae; h2ai; H2ak; H2AK5ac; H2AX histone; H2AZ histone; H2B histone family, member U, (testis-specific); HIS; HIST1H2AE antibody; HIST3H2A; histone 1, H2aa; histone 1, H2ab; histone 1, H2ad; histone 1, H2ae; histone 1, H2ag; histone 1, H2ah; histone 1, H2ai; histone 1, H2aj; histone 1, H2ba; histone 2, H2ai; histone 5 protein 2ax; Histone H2A type 1; Histone H2A type 1-A; Histone H2A type 1-B; Histone H2A type 1-B/E; Histone H2A type 1-C; Histone H2A type 1-D; Histone H2A type 1-E; Histone H2A type 1-G; Histone H2A type 1-H; Histone H2A type 1-I; Histone H2A type 1-J; Histone H2A type 1-N; Histone H2A type 1-O; Histone H2A type 1-P; Histone H2A.2; Histone H2A.3; Histone H2A.X; Histone H2A.Z; Histone H2A/a; Histone H2A/e; Histone H2A/g; Histone H2A/m; histone H2A/p; Histone H2A/ptl; Histone H2A/r; Histone H2A/s; histone H2AD; histone H2AE; Histone H2AX; Histone H2B type 1-A; Histone H2B, testis; MGC74460 antibody; Testis-specific histone H2B; TSH2B.1

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基因别名: 1190022L06; AW228881; bA317E16.2; bA317E16.3; dJ160A22.4; dJ86C11.1; gammaH2ax; H2a-291A; H2a-53; H2A.1; H2A.1b; H2A.2; H2A.3; H2A.X; H2A.Z; H2A.Z-1; H2A/a; H2A/c; H2A/E; H2A/g; H2A/m; H2A/p; H2A/S; H2A/X; H2A/z; H2AA; H2AC1; H2AC11; H2AC12; H2AC13; H2AC14; H2AC15; H2AC16; H2AC17; H2AC4; H2ac6; H2AC7; H2AC8; H2AFA; H2AFALii; H2AFC; H2AFD; H2AFE; H2AFG; H2AFI; H2AFM; H2AFN; H2AFP; H2AFR; H2AFX; H2AFZ; H2AG; H2AH; H2AX; H2AZ; H2AZ1; H2BC1; H2BFU; HIST1H2AA; HIST1H2AB; Hist1h2ac; HIST1H2AD; HIST1H2AE; HIST1H2AG; HIST1H2AH; HIST1H2AI; HIST1H2AJ; HIST1H2AK; HIST1H2AL; HIST1H2AM; Hist1h2an; Hist1h2ao; Hist1h2ap; HIST1H2BA; Hist5-2ax; pH2A/f; STBP; TH2A; TH2B; TSH2B; TSH2B.1