Invitrogen

Huntingtin Monoclonal Antibody (4-13)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他36个Huntingtin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

Huntingtin Antibody in Western Blot (WB)

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Huntingtin Antibody (MA1-058) in WB

Western blot was performed using Anti-Huntingtin Mouse Monoclonal Antibody (4-13) (Product # MA1-058) and a 62 kDa band corresponding to cleaved fragment of Huntingtin was observed across the tissues tested. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Kidney (Lane 2) and Mouse Liver (Lane 3) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0375BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). Th... View More

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产品信息

MA1-058

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化 (IHC)

2.5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

4-13

抗原

Synthetic peptide conjugated to KLH via cysteine corresponding to residues CSDPAMDLND (544-552) of Human HTT.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536730

产品详细信息

MA1-058 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry on human and mouse samples.

Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site.

Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody MA1-058 recognizes the 552 cleaved fragment without detecting the full-length form.

靶标信息

Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。