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Huntington Antibody (600-401-J23) in WB
Western Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 350 kDa for Huntington. Other band(s): Huntington splice variants and isoforms.
产品信息
600-401-J23
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
偶联物
形式
Liquid
浓度
1.1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
靶标信息
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
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生物信息学
蛋白别名: HD protein; HD protein homolog; Huntingtin; huntingtin (Huntington disease); Huntington disease gene homolog; Huntington disease protein; huntington disease protein homolog
基因别名: AI256365; C430023I11Rik; HD; Hdh; HTT; IT15
UniProt ID: (Human) P42858
Entrez Gene ID: (Human) 3064, (Rat) 29424, (Mouse) 15194
urea cycle
citrulline metabolic process
establishment of mitotic spindle orientation
protein import into nucleus
mitochondrial transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
apoptotic process
mitochondrion organization
endoplasmic reticulum organization
Golgi organization
dopamine receptor signaling pathway
spermatogenesis
gastrulation
central nervous system development
brain development
cell aging
learning or memory
learning
grooming behavior
locomotory behavior
axo-dendritic transport
associative learning
determination of adult lifespan
visual learning
anatomical structure morphogenesis
anterior/posterior pattern specification
endosomal transport
lactate biosynthetic process from pyruvate
quinolinate biosynthetic process
striatum development
olfactory lobe development
neural plate formation
neurogenesis
peptide hormone secretion
insulin secretion
positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
regulation of protein phosphatase type 2A activity
social behavior
hormone metabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
vesicle transport along microtubule
regulation of synaptic plasticity
paraxial mesoderm formation
animal organ development
neuron development
mRNA transport
neuron apoptotic process
response to calcium ion
regulation of mitochondrial membrane potential
L-glutamate import
iron ion homeostasis
negative regulation of neuron death
negative regulation of cysteine-type endopeptidase activity
negative regulation of extrinsic apoptotic signaling pathway
vocal learning
positive regulation of cilium assembly
embryo development
