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Invitrogen

ICAM-1 Monoclonal Antibody (MEM-111)

8 篇参考文献

查看其他109个ICAM-1 (CD54)抗体

说明书

实验方案

技术支持

货号 MA1-19123

CNY 4,332.00

100 µg

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说明书

实验方案

技术支持

抗体检测数据 (5)

Application

图: 1 / 5

ICAM-1 Antibody (MA1-19123) in WB

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-CD54 Monoclonal antibody (Product # MA1-19123) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88kDa.

ICAM-1 Antibody in Flow Cytometry (Flow)

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产品信息

MA1-19123

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

0.5-4 µg/mL

查看6篇已发表文章 6篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Rat

已发表种属

Cat, Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

MEM-111

抗原

Burkitt's lymphoma cell line Raji.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1072566

产品详细信息

This antibody reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Western Blot: non-reducing conditions. Immunohistochemistry (paraffin sections): positive tissue: thymus, RE cells.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.

Circulation research

Basehore SE,Bohlman S,Weber C,Swaminathan S,Zhang Y,Jang C,Arany Z,Clyne AM

MA1-19123 was used in Immunohistochemistry to test if steady laminar flow, but not oscillating disturbed flow, decreases eNOS O-GlcNAcylation, thereby elevating eNOS phosphorylation and NO production.

Fri Nov 12 00:00:00 UTC 2021

Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation.

Stem cell research & therapy

Taechangam N,Iyer SS,Walker NJ,Arzi B,Borjesson DL

MA1-19123 was used in Flow Cytometry to investigate the mechanisms used by feline adipose-derived mesenchymal stem cells (ASCs) to inhibit T cell proliferation, including the soluble factors and the cell-cell contact ligands responsible for ASC-T cell interaction.

Tue Jun 25 00:00:00 UTC 2019

Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting.

The American journal of pathology

Fromm JR,Thomas A,Wood BL

MA119123 was used in flow cytometry to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma

Wed Feb 01 00:00:00 UTC 2017

Integrated pathways for neutrophil recruitment and inflammation in leprosy.

The Journal of infectious diseases

Lee DJ,Li H,Ochoa MT,Tanaka M,Carbone RJ,Damoiseaux R,Burdick A,Sarno EN,Rea TH,Modlin RL

MA1-19123 was used in flow cytometry to identify genes involved in neutrophil recruitment in the lesions from patients with erythema nodosum leprosum

Mon Feb 15 00:00:00 UTC 2010

LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lee DJ,Sieling PA,Ochoa MT,Krutzik SR,Guo B,Hernandez M,Rea TH,Cheng G,Colonna M,Modlin RL

MA1-19123 was used in flow cytometry to test if leukocyte Ig-like receptor A2 regulates DC differentiation using a leprosy model.

Sat Dec 15 00:00:00 UTC 2007

Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.

American journal of clinical pathology

Fromm JR,Kussick SJ,Wood BL

MA1-19123 was used in flow cytometry to evaluate the use of flow cytometry to identify the Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma

Wed Nov 01 00:00:00 UTC 2006

Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.

The Journal of biological chemistry

Zhang F,Marcus WD,Goyal NH,Selvaraj P,Springer TA,Zhu C

MA1-19123 was used in flow cytometry to study conformational changes of the leukocyte integrin alphaLbeta2

Fri Dec 23 00:00:00 UTC 2005

Filarial antigens impair the function of human dendritic cells during differentiation.

Infection and immunity

Semnani RT,Sabzevari H,Iyer R,Nutman TB

MA1-19123 was used in flow cytometry to assess the effect of microfilariae antigen on dendritic cells

Sat Sep 01 00:00:00 UTC 2001

生物信息学

蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

基因别名: BB2; CD54; ICAM; Icam-1; ICAM1; P3.58

UniProt ID: (Human) P05362, (Bovine) Q95132, (Rat) Q00238

Entrez Gene ID: (Human) 3383, (Bovine) 281839, (Rat) 25464

功能

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding protein complex binding

参与通路

ICAM-1 Monoclonal Antibody (MEM-111)

ICAM-1 Antibody (MA1-19123) in WB

产品信息

MA1-19123

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

ICAM-1 Monoclonal Antibody (MEM-111)

ICAM-1 Antibody (MA1-19123) in WB

产品信息

MA1-19123

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID