Invitrogen

IDH1 Monoclonal Antibody (GT1521)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他26个IDH1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (10)
高级验证 (2)

IDH1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

IDH1 Antibody (MA5-27759) in ICC/IF

IDH1 Monoclonal Antibody (GT1521) detects IDH1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IDH1 protein stained by IDH1 Monoclonal Antibody (GT1521) (Product # MA5-27759) diluted at 1:500. Blue: Hoechst 33342 staining.

IDH1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

IDH1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with IDH1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-IDH1 Monoclonal Antibody (GT1521) (Product # MA5-27759). {KD}

产品信息

MA5-27759

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT1521

抗原

Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2735231

产品详细信息

Positive Control: 293T, A431, HeLa, HepG2, Huh-7, PC-12, Rat2, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12

Predicted Reactivity: Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Cytosolic NADP-isocitrate dehydrogenase; epididymis luminal protein 216; epididymis secretory protein Li 26; IDH; IDP; IDPc; isocitrate dehydrogenase 1 (NADP+); isocitrate dehydrogenase 1 (NADP+), soluble; Isocitrate dehydrogenase 1, soluble; Isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP+-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; OTTHUMP00000206464; OTTHUMP00000206465; OTTHUMP00000206466; Oxalosuccinate decarboxylase

查看更多收起

基因别名: AI314845; AI788952; E030024J03Rik; HEL-216; HEL-S-26; Id-1; IDCD; IDH; Idh-1; IDH1; IDP; IDPC; PICD

查看更多收起

UniProt ID: (Human) O75874, (Mouse) O88844, (Rat) P41562

查看更多收起

Entrez Gene ID: (Human) 3417, (Mouse) 15926, (Rat) 24479

查看更多收起

功能